STREX (black bars) and ZERO (open bars) mRNA levels expressed as a percentage of total BK channel transcripts in the respective tissue at each developmental time point. Splice variant expression was analysed in mouse: a) spinal cord, b) midbrain, c) cerebellum, d) pons and e) medulla at embryonic day 13 (E13), 15 (E15), 18 (E18) and postnatal days 7 and 35 (P7 and P35 respectively). All data are Means ± S.E.M, n = 5/tissue region. * p < 0.05, ** p < 0.01, compared to respective splice variant expression at P35, Kruskal-Wallis non-parametric test with post hoc Dunn's test for multiple comparisons.
Frameworks in the Diencephalon and you can Telencephalon
During the thalamus and you may hypothalamus a tiny, but high, rise in overall BK channel phrase are noticed out-of E15 so you’re able to P35 (Contour 3a 3b). On the other hand, complete BK route mRNA phrase enhanced almost 10-fold between embryonic and you can postnatal stages in frontal cortex, rear cortex, hippocampus, olfactory bulb, striatum and you can entorhinal cortex (Profile 3c–h). In every countries checked out, there is a life threatening developmental downregulation out of STREX version mRNA term (Contour 5). When you look at the front cortex, rear cortex, hippocampus, olfactory bulb, striatum and you can entorhinal cortex this really is on the a critical upregulation away from Zero version mRNA expression (Figure 5). Continue reading Developmental regulation away from STREX and Zero variation splicing during the structures out of the fresh new rhombencephalon, mesencephalon and back